Metabolic Alterations in Male Rats after Oral Exposure to Alcoholic Bitters
Abstract
Alcoholic bitters have been widely consumed in Nigeria for the management of several medical conditions such as hypertension, menstrual cramps, hormonal imbalance and sexual inadequacies, without adequate information on the consequent biochemical changes. This study was therefore carried out to assess the effects of selected alcoholic bitters on some biochemical parameters of the liver and kidney of male rats.
Twenty- five male Wistar rats were assigned into five groups (A-E) consisting of five animals each and administered 0.4, 0.2, 0.2, 0.16, and 0.3 ml/kg of water, ethanol, Alomo, Strikers and Orijin alcoholic bitters respectively for 28 days. The animals were sacrificed 24 hours after the last treatment and the liver and kidney were used for the determination of the concentrations of total protein, total cholesterol, triglyceride, malondialdehyde and the activities of β-Hydroxy β-methylglutaryl-CoA reductase, superoxide dismutase and catalase using standard methods.
The alcoholic bitters significantly reduced (p<0.05) the protein concentration in both the liver (26%) and kidney (23%) when compared with control. There were significant reductions (p<0.05) in the levels of total cholesterol and β-Hydroxy β-methylglutaryl-CoA reductase activity in both the liver and the kidney whereas the level of triglycerides was not significantly (p>0.05) altered. Malondialdehyde levels reduced significantly whereas activities of catalase and superoxide dismutase significantly increased (p<0.05) especially in the liver after the administration of the alcoholic bitters when compared with control. This study lends credence to the beneficial effects of alcoholic bitter as it may reduce hypercholesterolemia and enhance antioxidant status
Twenty- five male Wistar rats were assigned into five groups (A-E) consisting of five animals each and administered 0.4, 0.2, 0.2, 0.16, and 0.3 ml/kg of water, ethanol, Alomo, Strikers and Orijin alcoholic bitters respectively for 28 days. The animals were sacrificed 24 hours after the last treatment and the liver and kidney were used for the determination of the concentrations of total protein, total cholesterol, triglyceride, malondialdehyde and the activities of β-Hydroxy β-methylglutaryl-CoA reductase, superoxide dismutase and catalase using standard methods.
The alcoholic bitters significantly reduced (p<0.05) the protein concentration in both the liver (26%) and kidney (23%) when compared with control. There were significant reductions (p<0.05) in the levels of total cholesterol and β-Hydroxy β-methylglutaryl-CoA reductase activity in both the liver and the kidney whereas the level of triglycerides was not significantly (p>0.05) altered. Malondialdehyde levels reduced significantly whereas activities of catalase and superoxide dismutase significantly increased (p<0.05) especially in the liver after the administration of the alcoholic bitters when compared with control. This study lends credence to the beneficial effects of alcoholic bitter as it may reduce hypercholesterolemia and enhance antioxidant status
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