Indigenous commercial drinks as potential sources of extended spectrum β-lactamases (ESBLS) producing organisms in Kano, Nigeria

Abstract

Ten samples each of ten different indigenous commercial drinks were processed for bacterial isolation and Gram staining reactions. Gram negative isolates were subjected to biochemical tests for identification using standard procedures and confirmed enterobacteriaceae isolates were screened for extended spectrum β-lactamases (ESBLs) production using Clinical Laboratory Standards Institute (CLSI) breakpoint. Suspected ESBLs producers were subjected to Disc Diffusion Test (DDST) using standard discs of Augmentin {AUG 30μg (Optudisc)} and prepared discs of Ceftriaxone {CXN 30μg (Medireich)} and Ceftazidime {CAZ 30μg (Glaxo-Smithkline)}. Of the 80 samples processed, 11 (13.75%) yielded Gram negative isolates belonging to enterobacteriaceae family. These include; Citrobacter fruendii 2, Escherichia coli 1, Klebsiella pneumoniae 1, Morganella morganii 1, Proteus vulgaris 1, Salmonella typhi 2 and Serratia mercescens 3. Among the enterobacteriaceae isolates screened, the results of CLSI breakpoint test showed that 7 (36.36%) were ESBLs producers viz: Citrobacter fruendii 1, Escherichia coli 1, Morganella morganii 1, Serratia mercescens 2. On subjecting the CLSI positive isolates to DRM, only 4 (18.75%) were confirmed ESBLs producers. These include; Citrobacter fruendii 1, Escherichia coli 1 and Morganella morganii 1.