Effect of Vancomycin on Staphylococcus species isolated from clinical specimens in South-Western Nigeria

Abstract

Bacteriological samples, wound swabs, ear swabs, pus, abscess, blood, urine, wound aspirate, high vaginal swabs, aural swab and conjuctival swabs, were obtained from seventy individuals (males and females), fifty coagulase positive Staphylococcus species and twenty coagulase negative species of Staphykococcus.  The samples were drawn from patients attending University College Hospital (UCH), Ibadan; Obafemi Awolowo Teaching Hospitals, Ile-Ife, Ladoke Akintola University of Technology Teaching Hospital, osogbo and Ondo State Specialist Hospitals, Akure.  Organisms isolated are:  Staphylococcus aureus and Staphylococcus epidermidis.  All isolates were characterized biochemically and their sensitivity against Vancomycin was tested using agar diffusion technique while minimum inhibitory concentration was also determined.  All the isolates were susceptible to 30µg/disc of Vancomycin with diameter of zone of inhibition ranging from 15mm to 19mm.  The minimum inhibitory concentration (MIC) ranged between 0.5 µg/ml and 4.0µg/ml for coagulase positive isolates and between 0.5µg/ml for coagulase negative isolates.