In vitro callogenesis of Moringa oleifera Lam. using seed explant

Abstract

The use of plant growth regulators in tissue culture media is critical for callogenesis. The type and concentration of auxin and cytokinin used have profound effect on callus induction. In this study, three types of auxin and one cytokinin were investigated for media supplementation. The auxins were naphthalene acetic acid (NAA), indole acetic acid (IAA) and 2,4-dichlorophenoxyl acetic acid (2,4-D), each at concentrations of 0, 2.5, 5.0, 7.5 and 10 mg/l. The cytokinin used was benzyl amino purine (BAP) at concentrations of 0, 0.1, 1.0 and 10.0mg/l. The possible combination of each auxin and BAP were observed for the best growth regulator when used singly or in combination to supplement Murashige and Skoog (MS) medium for callogenesis of Moringa oleifera seed culture. Callus was observed in medium fortified with all three auxins when used singly and in combination. The most effective auxin was 2,4-D. The rate of callogenesis increased when the auxins were in combination with BAP at (0-1.0 mg/l). There was no formation of callus in the medium supplemented with BAP at 10 mg/l when used singly or in combination with the various auxins. The maximum callogenesis observed was in medium fortified with 2,4-D at 2.5 mg/l and BAP at 1.0mg/l.