Effect of Human Menopausal Gonadotropin on Rat Testes Damaged by Cisplatin Treatment

Abstract

Cisplatin is an established chemotherapeutic substance commonly used in the treatment of cancer. This study was conducted to examine the possible counter effects of Human Menopausal Gonadotropins (HMG) against testicular toxicity induced by Cisplatin in male rats. 20 adult male rats were equally divided into four groups; A, B, C and D which were the control, Cisplatin, HMG and Cisplatin+HMG groups respectively. Testosterone, Luteinizing Hormone (LH) and Follicle Stimulating Hormone (FSH) were analyzed using Enzyme Linked Immunosorbent Assay (ELISA) while the testes and epididymis were dissected out and fixed in Bouin’s fluid for histological preparation. A decrease in Testosterone, LH and FSH was observed in the (Cisplatin) group and group D (Cisplatin and HMG treatment) when compared to that of the control. Histological examinations in epidydimis and testes were further indication of significant damage to sertoli cells, leydig cells and germ cells populations induced by cisplatin. The adverse effect of Cisplatin was countered by the administration of HMG. In conclusion, Cisplatin-induced testicular toxicity was ameliorated by the administration of HMG.