Detection of icaD and MecA Genes and Antibiogram Profiling of Ear Swab Borne Staphylococcus aureus Isolates

Abstract

Bacteriological analysis of fifty (50) ear swabs, collected from fifty (50) consenting male undergraduate students was conducted using routine procedures which included pour plating. Thirty one (31) Staphylococcus aureus were tentatively identified and subjected to biofilm production test and antibiotic susceptibility assay using Congo red agar and disc diffusion procedure. The presence of   icaD and MecA genes students in the respective bacterial strains was ascertained using polymerase chain reaction and agarose gel electrophoresis. All the isolates were resistant to ceftazidime (100%), cefuroxime (100%), ceftriaxone (100%), cloxacillin (100%), and augmentin (100%), while all the isolates exhibited sensitivity towards gentamicin (100%).  Biofilm production was detected in only seven (7) of the thirty one (31) S. aureus strains. The amplified mecA gene was detected in  S. aureus isolates coded; 1,2,3, 4, 7 and 8  and  also, the amplified icaD gene was observed in  S. aureus isolates coded; 1,2,3, 4, 5, 6,  7 and 8 respectively.