Evaluating the Concept of Reducing Sugar (Glucose) and Growth in L-Asparaginase Production Using Some Selected Marine Actinomycetes

Abstract

Reducing sugar is a sugar that serves as a reducing agent. It falls under the category of carbohydrate or natural sugar but it consist of either a free aldehyde group (-CHO) or a ketone groups called the reducing end of the sugar. Microbial growth describes an increase in cell number of microorganisms provided with suitable nutritional and environmental conditions. This study evaluated the concept of reducing sugar and growth in L- asparaginase production using some selected marine actinomycetes. Different isolates of marine actinomycetes were screened for L- asparaginase production using rapid plate method and secondary screening was carried out by submerged fermentation using inoculum size of 5 % v/v, the inoculated. Czapek Dox broth was   incubated   at 30 ± 2 ^o C using a water bath shaker for 7 days at 120 rpm. Production of L -asparaginase was carried out  using Czapek Dox broth supplemented with L-asparagine (1 g/L), as production medium that was incubated at 30 ± 2 ºC for 7 days at 120 rpm. Optical density (growth rate) and reducing sugar concentration were determined spectrometrically at 600 and 540 nm, respectively. Actinosynnema mirum had the highest optical density of 1.965 ± 0.001 and Streptomyces coelicolor had the least optical density of 1.321±0.004. Streptomyces nobilis reached death phase on day 5 of production. The reducing sugar concentration reduced from 47.88±0.16 mg/ml to 6.77 ± 0.06 mg/ml for Actinosynnema mirum. Conclusively, appreciable amount of glucose concentration, reduced from day 0 to day 7 and growth of isolates in the course of production of L- Asparaginase followed a normal growth pattern, with Lag phase, growth phase, stationary phase and death phase.