Effect of AMP, ATP and 2-Mercaptoethanol on the Activity of Alcohol Dehydrogenase Isolated from Palm ((Elaies Guineensis)) and Raffia (Raphia Africana) Saps from Some Locations in Niger Delta Region of Nigeria

Abstract

Alcohol dehydrogenases (ADHs: EC.1.1.1.1) are the critical enzymes involve in the catalysis of the last step during alcohol production. Alcohol dehydrogenase, ADH was obtained from yeast of raffia (R. hookeri) and
palm ( E. guineensis) saps from selected polluted and non-polluted locations in Niger Delta region: Akwa Ibom, Bayelsa, Cross River, Delta, Edo, Imo and Rivers States. The activity of the ADH was determined at the temperature of 30 o C and pH 8.0. Adenosine monophosphate (AMP) activated the ADH from all the locations and highest enzyme activity was seen in ADH of yeast of E. guineensis from polluted site in Delta state. Adenosine triphosphate (ATP) and 2- mercaptoethanol inhibited the ADH from all the locations. Least reduction of activity in the presence of these inhibitors was observed in E.guineensis obtained from polluted environment in Delta state. The activity of ADH of Yeast isolated from either raffia or palm saps from polluted and non- polluted environment can be regulated by these modulators in industries involve in their uses such as in brewery and bakery.