The Effects of Hypoxia on Pattern and Pattern formation in cortical B50 Neuronal cells in culture

Abstract

Hypoxia has been implicated in nerve cell deaths that occur in a variety of neurological disorders and opioid receptor agonists have been shown to have some positive benefits on the nervous system. The aim of the present work was to investigate the effects of hypoxia and opioid receptor agonists’ treatment on the morphology of B50 neuronal cell lines cultured in hypoxia using neuronal pattern and pattern formation as a case study. The B50 cells were cultured in normal incubator (21%O₂; 5% CO₂) as control group and hypoxic incubator (5%O₂; 5% CO₂) as the experimental group, three opioid receptor agonists namely DAMGO (µ) DSLET (δ) and ICI-199,441 (κ) were administered to the cells for 48 hours as treatment against hypoxia after 48 hours of culture at dose of 10µM, 50µM and 100µM. Neuronal morphology and wellbeing was assessed using same field morphological assessment and lactate dehydrogenase leakage (LDH). The result showed groups of dead and degenerating B50 neuronal cells, altered neuronal pattern and pattern formation and some significant changes (P<0.05) in cellular levels of LDH leakage in normal B50 cells (100%), hypoxic cells (587%), and treated cells with 100μM DAMGO(μ) (143%), 50μM DSLET(δ) (140%) and 50μM ICI-199,441 (κ) (109%). The changes in morphology, neuronal pattern and LDH release indicate that hypoxia induced morphological and cellular changes in B50 cells in hypoxia and that B50 neuronal cells are susceptible to damage and injurious effects of hypoxia as most brain cells while opioid agonists have some potential benefits in the treatment of hypoxia-induced changes in neuronal B50 cells in culture.