Amelioration of nickel chloride-induced breast cancer in rats treated with allicin and Allium sativum extracts

Abstract

To analyse PRDX5 gene from induced breast cancer rats treated with Allium Sativum extracts, ninety female albino rats with average weight of 186.4g were classified into 6 groups namely NC, PC, NCAI, NCAII, NCAIII and NCGI, of 5 animals each. The experimental groups received different concentrations of both Allicin extract and NiCl2. The NC. which is Negative Control, received 20 mg/kg/bwt of NiCl2 only. While the PC, the Positive Control receive distilled water only. The other groups received different concentration of Allicin (50mg. 100mg and 200mg) and 20mg/kg/bwt of NiCl2. Animals treated with allicin extract has significant increase in body weight at the beginning of weeks 3 to 5. There was fluctuation in weight of group that receive the treatment NCAIII and NCGI. No significant differences were observed between NC and NiCl2 treated rats of NCGI, NCGII and NCGIII as the weight of the animals increased across the weeks. There was amplification in the PRDX5 gene located in breast tissue of rats. Rats treated with allicin and garlic extract shows a distinct band at 100 base pair. However, rats in the groups (NC, NCAI and NCAII) ID not have visible bands at 100bp due to the DNA damage caused by NiCl2. Rat treated with NCG II and III have a clear band. Histological assay revealed the presence of seromucous gland proliferation and atrophy with surrounding stromal fibrosis, ductal hyperplasia, ductal hyperplasia and intraductal proliferation with serous acinal hyperplasia and mucinous glands. The present study showed that allicin and garlic extract from A. sativum could be used as a preventive measure against NiCl2-induced breast cell proliferation in the breast tissues of female albino rat.